Method

Principle

Source

Sequencing

Direct read of sequence variation

Hybridization-based approaches - Heteroduplex melting

Denaturing gradient gel
Electrophoresis [DGGE]

Retarded heteroduplex mobility

in denaturing gradient

Myers & Lerman, 1987

Denaturing HPLC [dHPLC]

Accelerated heteroduplex mobility due to diminished affinity of partially 1993 melted DNA to hydrophobic immobile phase

Huber et al, 1993

DNA chip

Diminished or enhanced affinity to immobile DNA oligomers [“features”]

Pease et al 1994

DNA mismatch recognition

Chemical cleavage

Mobility of mismatched fragment

Cotton et al, 1988

Enzymatic cleavage

Youil et al, 1995

Mismatch protein binding

Affinity separation

Lishanski et al, 1994

Single-strand conformation

Single-strand conformation

Analysis [SSCP]

Altered mobility of self-hybridizing

ssDNA under non-denaturing conditions

Orita et al, 1989

Dideoxy fingerprinting

Altered mobility of multiple early chain-terminated pieces of ssDNA self-hybridizing under non-denaturing conditions

Sarkar et al, 1992